Efficacy of biomimicking chitosan scaffolds in bridging large nerve defect
- VernacularTitle:高仿真壳聚糖支架修复神经缺损的有效性研究
- Author:
Wei QU
;
Zhuojing LUO
- Publication Type:Journal Article
- Keywords:
nerve defect;
nerve scaffold;
biomimicking;
rat;
autograft implantation
- From:
Orthopedic Journal of China
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To develop biomimicking chitosan scaffolds with longitudinally oriented micro-channels,and investigate their efficacy in bridging 15 mm sciatic nerve gap in rats.[Methods]Chitosan scaffolds with longitudinally oriented micro-channels were fabricated using unidirectional freezing-dry methods.The chitosan scaffolds were used to bridge 15 mm nerve defect in rats,and their efficacy in bridging nerve gap was evaluated by morphometric analysis,retrograde labeling,electrophysiological studies and behavioral analysis.[Results]The chitosan scaffolds developed in the present study showed longitudinally oriented micro-channels,which resembled the dimensions of basal lamina channels in normal nerves.Implantation of chitosan scaffold achieved axonal regeneration and functional recovery similar to autograft implantation in vivo.[Conclusion]The chitosan scaffolds have inner microstructures which resemble the basal lamina channels in normal nerves.The chitosan scaffold may be used as an alternative to autograft in bridging nerve gaps.
