Genetic Damage of Human Bronchial Epithelial Cells Caused by Cigarette Smoke Combined with Nano-TiO_2
- VernacularTitle:香烟烟气与纳米二氧化钛对人支气管上皮细胞的遗传损伤研究
- Author:
Chun XIE
;
Geyu LIANG
;
Bing YE
- Publication Type:Journal Article
- Keywords:
Cigarette smoke extract;
Nano-TiO2;
DNA breakage;
Chromosomal aberration;
Single cell gel electrophoresis;
Micro-nuclear
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic damage of human bronchial epithelial cells (HBE) caused by cigarette smoke combined with nano-TiO2.Methods 16-HBE cells were exposed to cigarette smoke extract (CSE) and nano-TiO2(mg/L) at the doses of 0 mg/L CSE+0 mg/L nano-TiO2,50 mg/L CSE+0 mg/L nano-TiO2,75 mg/L CSE+0 mg/L nano-TiO2,100 mg/L CSE+0 mg/L nano-TiO2,0 mg/L CSE+10 mg/L nano-TiO2,50 mg/L CSE+10 mg/L nano-TiO2,75 mg/L CSE+10 mg/L nano-TiO2,100 mg/L CSE+10 mg/L nano-TiO2. After 24 h exposure,HBE viability was evaluated by the methyl thiazolyl tetrazolium (MTT) assay,single cell gel electrophoresis (SCGE,comet assay) was employed to assess DNA damage,chromosomal aberration was measured by micronucleus test. Results Except the group exposed to 10 mg/L nano-TiO2,HBE viability of the other treatment groups were lower than that of the control group and Comet % of the other treatment groups were higher than that of the control group (P
