Promotion effects of nerve regeneration of chemically extracted acellular nerve allograft in vitro
- VernacularTitle:化学去细胞异体神经促神经再生的体外实验研究
- Author:
Yu WANG
;
Jiang PENG
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
chemically extracted accllular nerve allograft;
electrophoretic;
dorsal root ganglion;
nerve regeneration
- From:
Orthopedic Journal of China
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]Electrophoretic analysis of rat chemically extracted acellular nerve allograft(CEAN) protein was performed to investigate the protein of CEAN.Dorsal root ganglia of chicken were cultured onto the surface of CEAN slice to observe the effect of CEAN structure on the fiber regeneration.[Method]The CEAN were perpared according to Sondell method and electrophoresed to observed whether the electrophoresis strip of 28-30kDa(Myelin Protein) exited or not.Dorsal root ganglia of chicken were cultured onto thc surface of CEAN slice and dyed with nerve fiber fluorescence to observe the orientation of nerve fiber growth on the surface of CEAN slice.[Result]Electrophoretic analysis of rat CEAN protein showed that the electrophoresis strip of 28-30 kDa totally disappeared.A large number of nerve fibers grew from DRG along the basement membrane of CEAN.[Conclusion]No myelin protein remained in the CEAN,while the basement membrane structure of CEAN can induce the nerve fiber to grow.
