Detection of CGRP receptor in rat bone marrow mesenchymal stem cells and its induced osteoblasts
- VernacularTitle:大鼠骨髓间充质干细胞成骨分化过程中降钙素基因相关肽受体的表达
- Author:
Zhao WANG
;
Dan JIN
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide;
calcitonin gene-related petide receptor;
bone marrow mesenchymal stem cells;
osteoblast
- From:
Orthopedic Journal of China
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To compare the expression of calcitonin gene-related peptide(CGRP) receptor in rat bone marrow mesenchymal stem cells(rBMSCs) and the induced osteoblasts.[Method]The rBMSCs were isolated using whole bone marrow adherence method,and classified into osteoblast-induced group and non-induced group.In the different periods of culturing(1,2,and 3 weeks),identification of osteoblasts was performed by immunohistochemistry and reverse transcriptase-polymerase chain reaction(RT-PCR),and expression of the CGRP receptor was detected by Western Blot and RT-PCR.[Result]RT-PCR test demonstrated that osteoblast-induced group had a higher expression of CGRP receptor than non-induced group at the same time point,expression of CGRP receptor was increased in a time-dependent manner in the osteoblast-induced groups.Western Blot test demonstrated that osteoblast-induced group had a higher expression of CGRP receptor than non-induced group at the same time point.Expression of CGRP receptor was increased in a time-dependent manner in osteoblast-induced groups.[Conclusion]According to the mRNA test and protein test,it is suggested that CGRP receptor exists in the rBMSCs.CGRP receptor expression increased in the progress of osteogenic differentiation.
