Effects of urinastatin on the expressions of NF-?B and TNF-?, liver transplantation in patients
- VernacularTitle:乌司他丁对肝脏移植早期外周血NF-?B和TNF-?及酶学活性的影响
- Author:
Shaobo ZHANG
;
Qian HE
;
Liang GE
;
Jianshe SHI
;
Gengjie WANG
- Publication Type:Journal Article
- Keywords:
Liver transplantation?Nuclear factor-?appa B?Tumor necrosis factor-alpha?Ischemia-reperfusion injury?Urinastatin
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of urinastatin on the expression levels of NF-?B and TNF-? liver transplantation in patients. Methods: Thirty-six patients of late stage hepatic cirrhosis were randomly divided into two eaquel groups: the experiment group was administrated by urinastatin 300 000 U and saline 10 mL via venous injection during liver transplantation, the control group was administrated 10 mL saline by venous injection at the same time. The blood samples were harvestd at 1, 2, 4 and 6 h after the blood recovery of donated liver. The nuclear factor-?B (NF-?B) P65 relative level of blood was detected by Western blot analysis and the TNF-? level of blood was detected by double antibody Sandwich enzyme linked immunosorbent assay. Serum ALT and AST were also measured. Results: The expression level of serum NF-?B p65, TNF-? in the experiment group were lower than those in the control group, and the difference was significant(P
