Research on genetic characteristics in the HA1 of influenza A(H1N1) viruses isolated in Taian City
- VernacularTitle:流感病毒泰山分离株H1N1亚型血凝素HA1基因特征分析
- Author:
Peiqiang HOU
;
Huili YANG
;
Juanjuan LU
;
Rongqiang ZHANG
;
Dayong TIAN
;
Yunqiang LIU
- Publication Type:Journal Article
- Keywords:
Influenza A virus;
Polymerase chain reaction;
Nucleotide sequence;
Antigen site
- From:
Chinese Journal of Disease Control & Prevention
2009;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the data of influenza A(H1N1) viruses surveillance and genetic characteristics from Taian City during 2005-2008,so a scientific basis can be provided for the prevention and treatment of influenza.Methods The specimens from Influenza-Like Illness(ILI) were collected.The viruses were isolated with MDCK cell and identified with HAI and RT-PCR.The product of PCR were sequenced.Then the sequences were analyzed through biometric software.Results A total of 121 influenza strains were obtained from 615 specimens,and 4 of them were identified as A(H1N1) subtype.There were 3 strains mutated on several sites.Compared with strains isolated in 2005,there were 5 and 8 mutations in the amino acid sequences of virus strains isolated in 2007 and 2008 respectively.And there were a total of 22 amino acid mutations compared with A/Brisbane/59/2007(H1N1).Conclusions Influenza type A(H1N1) are detected in Taian City.There are several mutations in the amino acid sequences of virus strains isolated in Taian. The antigenic drift of virus strains is due to accumulation of amino acid substitutions
