Effect of high energy shock waves on expression of BMP-2 in adult rabbits with necrotic femoral head
- VernacularTitle:高能震波促进缺血性坏死股骨头BMP-2的表达
- Author:
Huanzhi MA
;
Bingfang ZENG
;
Xiaolin LI
- Publication Type:Journal Article
- Keywords:
femoral head necrosis;
glucocorticoids;
high energy shock waves;
bone morphogenetic protein-2
- From:
Orthopedic Journal of China
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effect of high energy shock waves on the expression of BMP-2 in adult rabbits with necrotic femoral head,and to explore the mechanism of avascular femoral head necrosis treated with high energy shock waves.[Methods]Thirty eight male adult New Zealand white rabbits,weighting 3.0 to 4.0 kg(average weight 3.3 kg) were used to establish animal model of avascular femoral head necrosis.Bilateral femoral head necrosis was induced by methylprednisolone and lipopolysaccharide.The left hind limbs(treatment side) of all rabbits received shock wave treatment of the femoral heads and the right limbs(control side) of all rabbits received no shock wave treatment.The animals were killed at 24 hours and 1(n=6),2(n=6),4(n=6),8(n=6),and 12(n=6) weeks after shock wave therapy.The femoral heads were harvested and freed of soft tissue and cartilage.They were assigned to immunohistochemistry and quantitative real-time reverse transcriptase PCR.[Results]Compared with the contralateral control without shock wave treatment,the BMP-2 mRNA expression levels increased to a peak at 1 week after the shock wave treatment and remained high at 24 hours and 1、 2、 4、 8 weeks after the shock wave treatment.At 4,8 and 12 weeks after shock wave treatment,the average percentage of staining area of the femoral heads in the treatment side were significantly higher than those in the control side(P
