Design and Construction of G2 Glycoprotein Multi-epitope Antigen Gene of Hantavirus
- VernacularTitle:汉坦病毒G2糖蛋白多表位抗原基因的设计与构建
- Author:
Dongjing YANG
;
Jinying CHEN
;
Xu SU
- Publication Type:Journal Article
- Keywords:
Gene;
Hantavirus;
B cell epitopes;
Multi-epitope antigen
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the multi-epitope antigen(mea) gene of G2 glycoprotein of Hantavirus SEO type L99 strain.Methods The B cell epitopes was chosen and connected by three-peptide GPG after the amino acid sequence of G2 protein was analyzed and predicted by bioinformatical soft wares.The corresponding gene mea was constructed by overlap PCR,and cloned into the prokaryotic expression plasmid pET32a(+).Results The mea gene was successfully constructed by the five epitopes being chosen.The recombinant plasmid pET32a-mea was acquired by directional cloning.Conclusion The mea and its expression system E.coli BL21/pET32a-mea were constructed for the first time.The foundation was laid for the expression of the mea and its immunological applications.
