Effect of survivin antisense oligodeoxynucleotide on invasive ability of hilar cholangiocarcinoma cell FRH-0201
- VernacularTitle:Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭性的影响
- Author:
Meng ZHAO
- Publication Type:Journal Article
- Keywords:
Survivin antisense oligodeoxynucleotides?Hilar cholangiocarcinoma?Invasive?Matrix metalloproteinase-2
- From:
Chinese Journal of Current Advances in General Surgery
1998;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective: To study the inhibition effect of survivin antisense oligodeoxynucleotide (ASODN)on apoptosis and invasion of hilar cholangiocarcinoma cell line FRH-0201. Methods: ASODN was transfected into cholangiocarcinoma cell line FRH-0201 by LipofectamineTM2000. The level of MMP-2 and TIMP-2 in the supernatant was detected by enzyme linked immunosorbent assay (ELISA). The effect of ASODN transfection was observed by Transwell cell culture chamber. Results: The cell proliferation was inhibited in the ASODN group. The expression of MMP-2 in ASODN group was lower than those in the SODN and blank group (P
