Expression of OPG/OPGL protein and its significance in rat model of trauma-induced osteonecrosis of the femoral head
- VernacularTitle:骨保护素/骨保护素配体在大鼠创伤性股骨头坏死中的表达及意义
- Author:
Qijia LI
;
Chen PENG
;
Weidong LIANG
- Publication Type:Journal Article
- Keywords:
femoral head osteonecrosis;
osteoprotegerin;
osteoprotegerin ligand;
immunohistochemitry
- From:
Orthopedic Journal of China
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the expression of OPG/OPGL protein and its significance in rat model of trauma-induced osteonecrosis of the femoral head(ONFH). [Methods]Thirty-two SD rats about 6 months were divided randomly into experimental and control groups.The animal model of femoral head necrosis was established in 32 SD rats by removing round ligaments of femoral head.Animals were sacrificed at 1,2,4 and 6 weeks after operation,respectively.The specimens were examined through histological observation under light microscope.The other side with sham operation served as normal control group.The comparison of fat tissue with hematopoietic tissue in the cavity of bone marrow of femoral head were performed by CMIAS computer-assisted image and statistical analysis.The percentage of empty lacuna in the femoral heads was obtained.Immunohistochemistry was used to determine the expression of OPG/OPGL protein in ONFH.[Results]ONFH was confirmed in experimental group.The model in various stages was successfully duplicated.Compared with normal control group,the percentage of empty lacuna remarkable increase was found in experimental groups in different periods(P
