Cytotoxicity of human doppel protein and Dpl-like protein PrP?32-121 to SH-SY5Y cells
- VernacularTitle:人doppel蛋白及其类似蛋白PrP?32-121对SH-SY5Y细胞的细胞毒性作用
- Author:
Kun XU
;
Xin WANG
;
Chan TIAN
;
Song SHI
;
Guirong WANG
;
Qi SHI
;
Ruimin ZHOU
;
Huiying JIANG
;
Yonglie CHU
;
Xiaoping DONG
- Publication Type:Journal Article
- Keywords:
doppel protein;
PrP;
cytotoxicity;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the biological activities of human doppel(Dpl) protein transiently expressed and Dpl-like protein PrP?32-121 on a human neuroblastoma cell line SH-SY5Y.Methods Recombinant mammalian expression plasmids containing human PRND gene and truncated PrP?32-121 fragment were generated by PCR.The expression and location of Dpl and PrP?32-121 post-transfection were observed by IFA.The cytotoxicity was measured by MTT analysis.Cellular apoptosis was investigated by flow cytometry and Western blot.Results Both Dpl and PrP?32-121 protein were expressed and mainly located on the cell membrane.Remarkable cytotoxicity was detected on SH-SY5Y cells after 24 h transfection.Meanwhile,more Annexin V/PI positively-stained cells as well as lower levels of cellular pro-caspase-3 and Bel-2 were detected in the cells receiving Dpl and PrP?32-121 expressing plasmids.Conclusion Dpl protein transiently expressed and PrP?32-121 can lead to the similar neural cytotoxicity,probably triggering the cell apoptosis program.
