COX-2 modulates ?~9-THC-induced inhibition of LTD in CA1 area
- VernacularTitle:环氧合酶-2介导四氢大麻酚抑制CA1区长时程抑制的作用
- Author:
Hongwei YANG
- Publication Type:Journal Article
- Keywords:
cyclooxgense-2(COX-2);
?9-THC;
long-term depression(LTD);
synaptic transmission;
hippocampus
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of cyclooxgense-2(COX-2) in tetrahydrocannabinol(THC)-induced inhibition of long-term depression(LTD) in CA1 area in vitro.Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of ?9-THC(10 mg/kg) or COX-2 inhibitor NS398(10 mg/kg).Field excitatory post-synaptic potentials(fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398,a selective inhibitor of COX-2,on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation(1 Hz,15 min) induced-LTD in CA1 area was significantly attenuated by ?9-THC.② ?9-THC did not affect the basal synaptic transmission and membrane excitability(including membrane resting potentials,input resistance and firing property).③ THC-induced inhibition of LTD was reversed by NS398.④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice.Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.
