Effects of Fluoride and Aluminum Alone and in Combination Exposure on Proliferation and Cell Cycle of MC3T3-E1 Cells
- VernacularTitle:氟铝单独及联合染毒对MC3T3-E1细胞增殖能力及细胞周期的影响
- Author:
Ruoxin CAI
;
Xiaoying GUO
;
Xin ZHAO
- Publication Type:Journal Article
- Keywords:
Fluoride;
MC3T3-E1 cells;
Aluminium;
Proliferation;
Cell cycle
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different concentrations of fluoride, aluminum alone and in combination exposure on mice parietal bone cell subclone 14 (MC3T3-E subclone 14), and to elucidate the pathogenesis of endemic fluorosis. Methods The proliferation of MC3T3-E1 cells exposed to 10-9 -10-3 mol/L NaF alone, 50 ?mol/L NaF and 5 ?mol/L AlCl3 alone and in combination ,was measured by CCK-8, and the change of cell cycle was measured by flow cytometry after treatment with various concentrations of fluoride and aluminum. Results Fluoride alone did not promote osteoblast MC3T3-E1cells proliferation, higher concentration fluoride inhibited MC3T3-E1 cells proliferation. Fluoride and aluminum combined exposure (50 ?mol/L NaF +5 ?mol/L AlCl3) stimulated proliferation of MC3T3-E1 cells (P
