Detection of Legionella pneumophila rcp Virulence Gene by Polymerase Chain Reaction
- VernacularTitle:军团菌rcp毒力基因的聚合酶链反应检测方法研究
- Author:
Lingqi YU
;
Dongjing YANG
;
Wen LI
- Publication Type:Journal Article
- Keywords:
Legionella;
rcp virulence gene;
Polymerase chain reaction
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a polymerase chain reaction method for the detection of the rcp virulence gene of L. pneumophila. Methods Sixteen strains of Legionella pneumophila, L. dumiffii, L. bozemanii and L. Longbeachae isolated from the cooling towers of the centralized air-conditioning systems in Tianjin form 2005 to 2007 were detected by polymerase chain reaction method using L. pneumophila rcp virulence gene-specific primer according to GenBank published nucleotide sequence. Results The 900 bp rcp genetic fragment was amplified in three strains of L. pneumophila, while others not. Conclusion The rcp virulence gene-based polymerase chain reaction method for the detection of L. pneumophila has been successfully established and is the foundation for the studies of Legionella virulence.
