Effects of Folate on Sodium Arsenite-Induced Toxicity and Oxidative Stress in Chang Hepatocytes
- VernacularTitle:叶酸对亚砷酸钠致Chang肝细胞毒性和氧化应激的影响
- Author:
Yuanyuan XU
;
Huihui WANG
;
Gexin LI
- Publication Type:Journal Article
- Keywords:
Arsenic;
Folate;
Oxidative stress;
Apoptosis
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different folate levels on inorganic arsenic-induced cytotoxicity and oxidative stress status. Methods Chang human hepatocytes were cultured with 20 ?mol/L sodium arsenite (NaAsO2) in medium containing 0 ?mol/L, 2.3 ?mol/L and 10 ?mol/L of folate, respectively. After 24 h of treatment with NaAsO2, cell viability was analyzed with 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis was assessed by Annexin V/PI double staining, levels of ROS were detected by 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) probe, and intracellular reduced glutathione (GSH) and malondialdehyde (MDA) content were determined by DTNB method and formation of thiobarbituric acid reactive substances, respectively. Results The decrease of cell viability and the increase of intracellular ROS and MDA induced by NaAsO2 were significantly exacerbated by folate deficiency but attenuated by folate supplement (P
