Effects of Sodium Arsenite on Expression of Transcription Factor Nrf2 and Heme Oxygenase 1 in Chang Liver Cells
- VernacularTitle:亚砷酸钠对核转录因子Nrf2和血红素单加氧酶-1表达的影响
- Author:
Bing LI
;
Jie LIU
;
Xin LI
- Publication Type:Journal Article
- Keywords:
Arsenic;
Sodium arsenite;
Nuclear factor (erythroid-2 related) factor 2;
Heme oxygenase 1
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of sodium arsenite (NaAsO2) on the expression of transcription factor nrf2 (Nrf2) and heme oxygenase 1 (HO-1) in Chang liver cells, and to explore the possible mechanism by using buthionine sulfoximine ( BSO), a GSH synthesis inhibitor. Methods Chang liver cells were treated with NaAsO2 at the doses of 5, 10 and 20 ?mol/L, alone, for 24 h, or pretreated with BSO (3 mmol/L, 12 h). Western blot assays were used to detect the protein expression of Nrf2 and HO-1. Results The protein expression of Nrf2 and HO-1 significantly increased in 5, 10 and 20 ?mol/L of NaAsO2 alone groups, and Nrf2 and HO-1 protein expression was up-regulated even higher by BSO pretreatment (P
