Primary Culture of Osteoblasts of Neonatal Mice:a Comparative Research
- VernacularTitle:新生小鼠成骨细胞原代培养方法的实验研究
- Author:
Ruoxin CAI
;
Xiaoying GUO
;
Guifan SUN
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Collagenase;
Primary culture
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm an alternative method for primary culture of osteoblasts through comparison of two primary culture methods. Methods Calvarias were dissected from newborn Kunming mice, osteoblasts were isolated with serum-containing collagenase digestion method and sequential digestion method respectively. The osteoblasts were observed under invert microscope, the growth curve was made with the application of MTT method, the rate of living osteoblasts was counted with trypan blue method respectively. Results Compared with the serum-containing collagenase digestion method, the sequential digestion method presented higher production of osteoblasts, higher cell survival rate (P
