Effect of resveratrol combined with DDP on FRH-0201 cholangiocarcinoma cell line
- VernacularTitle:白藜芦醇联合顺铂对FRH-0201细胞的影响
- Author:
Hui FU
;
Xiaopeng WU
;
Sen GUO
;
Rui ZHAO
;
Ran TAO
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms?Resveratrol?Cisplatin
- From:
Chinese Journal of Current Advances in General Surgery
2009;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objcetive: To investigate the effect of resveratrol (RES)combined with cisplatin (DDP)on the cholangiocarcinoma cell line FRH-0201 in vitro. Methods: FRH-0201 cells were exposed to different concentrations of RES or (and)DDP. Cell morphological changes were observed by light and fluorescence microscopy. Inhibition of cell proliferation was measured by MTT and colony-forming assay. And the flow cytometry were performed to detect the cell apoptosis rate and cell cycle. Results: Resveratrol inhibited the proliferation of FRH-0201 cells in a time-and dose-dependent manner in the concentration of 5～320 ?mol/L and the IC50 values after the exposure of 24, 48, and 72 h were 55.35, 32.84, and 28.01 ?mol/L respectively. RES and DDP had synergetic effect in the inhibition of FRH-0201 cells. Conclusion: RES can inhibit the growth of FRH-0201 cells and has the synergetic effect with DDP.
