Role of TNF-? in the mechanism of hepatocyte apoptosis induced by intestinal perforations due to abdominal firearm wound
- VernacularTitle:猪腹部火器伤肠管穿透后TNF-?在肝细胞凋亡中的作用
- Author:
Zexin LI
;
Xiao WANG
;
Huichao XUE
;
Jiangwei LIU
;
Yongjiu ZHANG
- Publication Type:Journal Article
- Keywords:
Abdominal injuries ?Firearm wound ?Hepatocyte apoptosis ?Endotoxin ?Tumor necrosis factor alpha?Swine
- From:
Chinese Journal of Current Advances in General Surgery
2009;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of TNF-?in the mechanism of hepatocyte apoptosis induced by intestinal perforations due to abdominal firearm wound. Methods:A total of 42 Chang-Bai piglets were divided randomly into 7 groups: control group and wounded 1, 2 , 4, 8, 12, 24 hour groups.The model of intestinal perforations due to abdominal firearm wound were established in wounded groups. Levels of plasma endotoxin were measured using ehromogenic limulus amehoeyte lysate test.Hepatic TNF-? content was measured with immunohistochemical staining and image analysis in all groups. Hepatocyte apoptosis indexes and serum TNF-? levels were determined at the same time. Results: Levels of plasma endotoxin, serum TNF-?, hepatic TNF-? content and hepatocyte apoptosis indexes in wounded groups were all significantly elevated compared with control group(P
