Inducing bone mesenchymal stem cells of rabbits into chondrocytes using the technology of micromass culture in vitro
- VernacularTitle:体外微团培养兔骨髓间充质干细胞形成软骨细胞
- Author:
Bin LI
;
Wei ZHANG
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
bone mesenchymal stem cells;
chondrocytes;
transforming growth factor-?1;
insulin-like growth factor-Ⅰ;
basic fibroblast growth factor
- From:
Orthopedic Journal of China
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To study the methods of inducing bone mesenchymal stem cells(BMSCs)of rabbits into chondrocytes in vitro and the interaction of transforming growth factor ?1(TGF-?1),insulin-like growth factor-Ⅰ(IGF-Ⅰ)and basic fibroblast growth factor(bFGF).[Methods]BMSCs of rabbits were primarily cultured and subcultured in vitro,and then divided into four groups according to the difference of factors:group A receiving TGF-?1 and bFGF;group B receiving TGF-?1 and IGF-Ⅰ;group C receiving TGF-?1;group D receiving no cell growth factor.After three weeks all the four groups were detected by methyl thiazolyl tetrazolium(MTT)assay,measurement of glycosaminoglycan(GAG)and immunohistochemistry.[Results]Immunohistochemical detection of collagen Ⅱ was positive in groups A,B and C.The results of the MTT assay and the GAG content in groups A and B were obviously higher than those in groups C and D.[Conclusion]Rabbit BMSCs can be induced into chondrocytes under certain conditions.TGF-?1,IGF-Ⅰ and bFGF have synergy effect in the differentiation from BMSCs into chondrocytes.
