Simultaneous Determination of Six Kinds of Phthalic Acid Esters in Human Serum by Gas Chromatography-Mass
- VernacularTitle:人体血清中6种邻苯二甲酸酯的气相色谱-质谱同时测定法
- Author:
Chunying LIANG
;
Hongmei DENG
;
Yongheng CHEN
- Publication Type:Journal Article
- Keywords:
Chromatography,gas;
Mass spectrometry;
Serum;
Phthalic acid esters
- From:
Journal of Environment and Health
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to determine six kinds of PAEs in human serum simultaneously,and on this basis to understand the level of PAEs in human body.Methods Ultrasonic extraction,gas chromatography-mass spectrometry-selected ion monitoring(GC-MS-SIM) detection and quantitative analysis based on internal standard were used to detect six kinds of PAEs in human serum simultaneously.Finally this method was used in fifty human serum samples.Results The results showed that this method had a good linear relation in the range from 10 to 1 000 ng/ml,and the correlation coefficients of standard curve were higher than 0.999.The average recovery rate and the relative standard deviation(RSD,n=6) of the target compounds in standard-addition blank was 94.5% and 4.4% respectively,the lower limits of detection for DEP,DMP,DBP,BBP,DEHP,DNOP were 0.31,0.85,0.90,0.63,0.88,0.41 ng/ml respectively,and the recovery rates of all samples ranged from 67.98% to 110.8%.Conclusion This method has good recovery rate,reproducibility,lower limit of detection and can be used for the determination of many kinds of PAEs substances in human serum.
