Detection of Common Fungal Pathogens by Genenral Primer PCR
- VernacularTitle:常见致病真菌通用引物PCR检测技术研究
- Author:
Min JIN
;
Aihua HUANG
;
Zhaoli CHEN
- Publication Type:Journal Article
- Keywords:
Fungi;
General primer PCR;
Detection
- From:
Journal of Environment and Health
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the detection method of common fungal pathogens by general primer PCR.Methods The primers were designed from the target genes of 5.8S rDNA and 28S rDNA of fungi,and the specificity and sensitivity were observed.Results The general primer PCR can be used to detected C.albicans,C.parapsilosis,C.krusei,C.glabrata,C.tropicalis,C.neoformans,A.fumigatus,A.flavus,A.nidulans,A.niger,C.carrionii,P.verrucosa,S.schenckii,F.pedrosoi,T.rubrum,T.mentagrophytes,M.gypseum,M.canis,E.floccosum,M.racemosus,the sensitivity was 15 pg/ml of DNA.Conclusion The general primer PCR can be used to detect common fungal pathogens.
