Effect of cerium nitrate on activity of the enzyme related to energy metabolism,cell cycle and apoptosis of testiculus cell in male mice
- VernacularTitle:硝酸亚铈对小鼠睾丸细胞能量代谢相关酶活性、细胞周期及凋亡的影响
- Author:
Haijun ZHAO
;
Baogui WANG
;
Guiying ZHANG
;
Hui ZHANG
;
Jing ZHOU
;
Feng LING
;
Minhua CHEN
- Publication Type:Journal Article
- Keywords:
cerium nitrate;
cell cycle;
apoptosis;
reproduction toxicity
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cerium nitrate on activity of the enzyme related to energy metabolism,cell cycle and apoptosis of testiculus cell in male mice.Methods A total of 100 male mice were distributed randomly into negative control group,cytoxan group,cerium nitrate groups in low dose(15mg/kg),medium dose(30mg/kg),high dose(60mg/kg).Whole mice were given corresponding reagent for 60 days.Enzyme-chemical method was used to detect the activity of lactate dehydrogenase(LDH),sorbitol dehydrogenase(SODH),succinic dehydrogenase(SDH),glucose-6-phosphate dehydrogenase(G-6PD).Cell cycle and apoptosis of testiculus cell were studied by the way of one-fluorescence dying method on flow cytometer.Results 15mg/kg cerium nitrate could promote the activity of LDH,SODH,SDH and G-6PD of testiculus cell compared with that of cytoxan(P
