Effects of high glucose on the proliferation of HSC and expressions of TGF-?_1,PDGF and PCⅢ in HSCs
- VernacularTitle:高糖对肝星状细胞增殖及对转化生长因子?_1、血小板衍化生长因子和Ⅲ型前胶原表达的影响
- Author:
Mingli JIAO
;
Li REN
;
Xinming CHANG
- Publication Type:Journal Article
- Keywords:
hepatic stellate cell(HSC);
transformation growth factor(TGF);
platelet-derived growth factor(PDGF);
precollagen Ⅲ(PCⅢ)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of high glucose on the proliferation of hepatic stellate cell-T6(HSC-T6) and the expressions of transformation growth factor-?1(TGF-?1),platelet-derived growth factor(PDGF) and precollagen Ⅲ(PCⅢ).Methods Based on glucose groups of different concentration,we observed the proliferation of HSC in 30min-16h time period,and used the methods of immunohistochemistry and radioimmunoassay to measure the expressions of TGF-?1,PDGF and PCⅢ in the supernatant at 48h and 72h.Results In 2-16h time period,the proliferation of HSC was increased stepwise over time in 1500-6000mg/L group,and was more visible in 4500-6000mg/L group.The expressions of TGF-?1 and PDGF increased at 48h,and the expression of PCⅢ in the supernatant increased at 72h in 4500-6000mg/L group compared with that in glucose control and hypertonic control groups.Conclusion High glucose can promote hepatic fibrosis by stimulating the expressions of TGF-?1,PDGF and PCⅢ in HSC-T6.
