VEGF antisense oligodeoxynucleotides in enhancing the apoptosis-inducing effect of Cinobufotalin on K562 cells
- VernacularTitle:血管内皮生长因子反义核酸增强华蟾素诱导K562细胞凋亡
- Author:
Limei CHEN
;
Chaomin LI
;
Huaiyu WANG
;
Jianhong GUAN
- Publication Type:Journal Article
- Keywords:
Cinobufotalin;
VEGF antisense oligonucleotide;
K562 cell;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the vascular endothelium growth factor(VEGF) antisense oligonucleotide(ASON) Cinobufotalin in enhancing apoptosis induced by human chronic myeloid leukemia K562 cell line.Methods The synthesis of VEGF ASON was transfected into the K562 cells;Cinobufotalin of four concentrations(1,3,5 and 7mg/L) acted on the K562 cell line for 24h,48h and 72h.Western blot was used to detect VEGF protein expression,while in situ apoptosis(TUNEL) and flow cytometry method(FCM method) were employed to detect the apoptosis.Results The different doses of Cinobufotalin all inhibited K562 cell line in time-and dose-dependent manners.K562 cell line transfected by VEGF ASON had a more pronounced induction of apoptosis.Conclusion The in vitro Cinobufotalin at a certain range of concentration can induce apoptosis in K562 cell line,and VEGF ASON enhances Cinobufotalin's effect in inducing apoptosis of K562 cells.
