CRYOGENIC PRESERVATION OF ERYTHROCYTES USING DIMETHYLSULFOXIDE (DMSO)
- VernacularTitle:二甲基亚砜低温冷冻红细胞
- Author:
Jinghan LIU
;
Yufeng HAN
- Publication Type:Journal Article
- Keywords:
Dimethylsulfoxide Erythrocytes Cryogenic preservation
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Erythrocytes were frozen with DMSO of different concentrations (not over 20%) as a cryoprotec-tive agent in freezers of different temperatures.The rate of temperature drop was not controlled. Of the erythrocytes actually frozen, the average recovery rate after thawing was 91.3 per cent (range 87.5~ 95%). The average survival rate of erythrocytes stored at -20℃, -30℃, or -80℃ for up to eight months was 90.9 per cent (range 88.2~ 94.5). As compared with glycerol, which is in common use internationally, DMSO seems to be a better cryoprotective agent.
