Effect of cisplatin on ototoxicity and expression of caspase-3 in mouse cochlea
- VernacularTitle:顺铂对小鼠耳蜗毒性及caspase-3表达的影响
- Author:
Weixia SUN
;
Aimei WANG
;
Cuifen BAO
;
Lijie HUI
;
Zhijie CHANG
- Publication Type:Journal Article
- Keywords:
cisplatin;
cochlea;
caspase-3;
immunohistochemistry
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mice model of cisplatin-induced ototoxicity and to investigate the effect of cisplatin on the expression of caspase-3 in mouse cochlea.Methods Totally 69 Kunming mice were randomly divided into control group,cisplatin 2.5mg/(kg?d) group,cisplatin 3.5mg/(kg?d) group and cisplatin 4.5mg/(kg?d)group.Mice were injected intraperitoneally for 5 days.Auditory brainstem response(ABR) was measured to observe the change of hearing.Envision method of immunohistochemistry was applied to detect the expression of caspase-3 in cochlea.Results The weight and hearing of mice in different dose cisplatin groups were declined significantly as compared with those in control group(P
