Comparison of Four Commercial ELISA Kits and In-House Immunoblotting for Diagnosis of Helicobacter pylori Infection.
10.5223/pghn.2012.15.2.85
- Author:
Hoar Lim JEONG
1
;
Yang Sook JUNG
;
Jin Su JUN
;
Jung Sook YEOM
;
Ji Sook PARK
;
Ji Hyun SEO
;
Jae Young LIM
;
Chan Hoo PARK
;
Hyang Ok WOO
;
Hee Shang YOUN
;
Gyung Hyuck KO
;
Seung Chul BAIK
;
Woo Kon LEE
;
Myung Je CHO
;
Kwang Ho RHEE
Author Information
1. Department of Pediatrics, Gyeongsang National University School of Medicine, Institute of Health Science, Jinju, Korea. hsyoun@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
ELISA;
Immunoblot;
Helicobacter pylori infection;
Children
- MeSH:
Adult;
Aged;
Antibodies;
Child;
Enzyme-Linked Immunosorbent Assay;
Helicobacter;
Helicobacter pylori;
Humans;
Immunoblotting;
Immunoglobulin G;
Seroepidemiologic Studies;
Serologic Tests
- From:Pediatric Gastroenterology, Hepatology & Nutrition
2012;15(2):85-90
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Commercial enzyme-linked immunosorbent assay (ELISA) kits have been considered less reliable for children than for adults. The aim of this study was to compare four ELISA kits and in-house immunoblotting based on the analysis of anti-H. pylori-IgG antibody reactivity. METHODS: A total of 399 serum samples were collected at the GNU Hospital during 1998-1999. All sera were tested using ELISA and immunoblotting. Statistically significant differences were determined by the chi2 test. RESULTS: The overall seropositivity rates using GAP IgG, Genedia IgG, HM-CAP, Pyloriset EIA-G, and immunoblotting were 13.0%, 25.1%, 18.3%, 15.8%, and 62.9%, respectively. Immunoblotting showed a higher seropositivity rate than did all four ELISA kits in all age groups. Genedia IgG had the highest seropositivity among the ELISA kits. The seropositivity rate for children aged 13 to 18 months was lowest, and that of children aged 15 years was highest (90.0%). The seropositivity rate for children aged 7 months to 5 years was significantly lower than that for children aged 6 to 15 years among the four ELISA kits (p<0.0001) and immunoblotting (p=0.02). CONCLUSION: Immunoblotting is the most sensitive test for detection of anti-Helicobacter pylori IgG antibodies among the serological tests in this study. These results emphasize the need for standardization when commercial ELISA tests are used in different nations or in young age groups. Immunoblotting could be a suitable noninvasive assay for serodiagnosis and seroepidemiologic study of H. pylori infection in Korean children.
