The mRNA expression of TLR4,TLR9 in experimental autoimmune neuritis induced by P0 180-199 and effects of TWP on it
- VernacularTitle:TLR4,TLR9 mRNA在P0 180-199诱导Lewis大鼠EAN模型中的动态表达及TWP对其的影响
- Author:
Yongning DENG
;
Wenbin ZHOU
;
Guoxiang HUANG
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune neuritis(EAN);
TLR (Toll like receptor);
APC (Antigen presenting cell);
TWP (Tripterygium wilfordii Polycoside)
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the dynamic expression of mRNA of TLR4 and TLR9 in Lewis rats with experimental autoimmune neuritis(EAN) and the effect of TWP on the disease.Methods:Male Lewis rats were immunized with P0 180-199(100 microgram),TWP was profused into post-immunization rats’ stomach daily.The clinical signs of rats and pathological changes in the sciatic nerves were observed.TLR4 and TLR9 were detected by RT-PCR dynamically which spleens,sciatic nerves and peripheral blood lymphonodes as sample.Results:EAN group got the peak of clinical score at the 17 d.p.i,and ameliorated obviously at 33 d.p.i,and the mRNA expression of TLR4 got the peak at the 16 d.p.i,then reduced gradually(P
