Vaccination of mice with B16 cells expressing the CRT-E2 fusion protein induces anti-tumor immune responses
- VernacularTitle:表达CRT-E2融合蛋白肿瘤疫苗诱导特异性抗肿瘤免疫应答
- Author:
Jianlin YANG
;
Danli LI
;
Changbai LIU
- Publication Type:Journal Article
- Keywords:
Calreticulin;
HPV E2;
Tumor vaccine
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the anti-tumor effects by vaccination of mice with murine melanoma B16 cells expressing calreticulin (CRT) and Human papillomavirus 16 E2 fusion protein.Methods:Mice were inoculated with B16 cells expressing CRT,E2,or CRT-E2 by intraperitoneal injection. The inoculation was repeated after one week. The activity of CTLs and NK cells,the proliferation of T cells and the amount of IFN-? secreted by spleen lymphocytes were analysed.Results:The activity of CTLs and NK cells,and the amount of IFN-? secreted by spleen lmyphocytes from mice vaccinated with CRT-E2 fusion protein were significantly higher than those of the other groups(P
