Induction,proliferation and functional study of specific anti-acute myeloid leukemia(AML) T cells
- VernacularTitle:抗AML特异性T细胞的诱导、扩增及功能研究
- Author:
Nian LIU
;
Xiaoying QIN
;
Bin JIANG
- Publication Type:Journal Article
- Keywords:
Aute myeloid leukemia (AML);
Dendritic cell (DC);
T lymphocyte;
Immunotherapy
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop specific anti-AML T cells in vitro,and to study their biological characteristics and functions.Methods:Peripheral blood or bone marrow mononuclear cells (MNCs) were isolated from 12 patients with AML,and co-cultured with cytokine combinations in 96 well plates to be induced into dendritic cells (DCs).Cell morphology was observed under an inverted microscope during the first week and immunophenotype was detected by flow cytometry at day 7.Cytokine combination was replaced with high dose IL-2 at day 7 to promote specific anti-AML T cells.T cell phenotype was detected after 4 to 5 weeks.Lactate dehydrogenase (LDH) release assay was used to determine T cell killing activity.Results:After being cultured with cytokine combinations,the typical dendritic appearance with delicate membrane projections was observed.CD80,CD86 and HLA-DR markers were significantly upregulated(P
