Determination of Three Kinds of Anti-dandruff Agents in Cosmetics by High Performance Liquid Chromatography
- VernacularTitle:化妆品中3种去屑剂的高效液相色谱测定法
- Author:
Liming CHEN
;
Guosheng HU
;
Yuping TIAN
- Publication Type:Journal Article
- Keywords:
Cosmetics;
Chromatography,liquid;
Anti-dandruff agent
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for simultaneous determination of three kinds of anti-dandruff agents,namely ketoconazole,climbazole,piroctone olamine,in cosmetics by HPLC.Methods The cosmetics were extracted with acetonitrile by ultrasonic,then separated with high-speed centrifuge.The supernatant layer was filtrated through membrane with 0.45 ?m diameter.The filtration liquid was injected into HPLC for detection at ?=210 nm.Quantitative analysis was performed with external standard method.Results In the linear range of 1-250 mg/L,the regression equation of ketoconazole was y=32.23x+ 4.59,the detection limit was 0.069 mg/L.In the linear range of 1-500 mg/L,the regression equation of climbazole was y=13.76x+ 9.41,the detection limit was 0.12 mg/L.In the linear range of 10-500 mg/L,the regression equation of piroctone olamine was y= 24.83x-17.17,the detection limit was 0.71 mg/L.The recoveries and relative standard deviations were 91.8%-98.5% and 0.38%0.84%,respectively.Conclusion The experimental results show that the method is simple,precise and accurate and suitable for simultaneous determination of 3 kinds of anti-dandruff agents in cosmetics.
