DNA Damage in Testicular Cells Induced by Acrylamide through Different Exposure Routes in Mice
- VernacularTitle:三种方式染毒丙烯酰胺对雄性小鼠睾丸细胞DNA损伤的影响
- Author:
Xiaoling ZHANG
;
Wenjuan WANG
;
Yuan LI
- Publication Type:Journal Article
- Keywords:
DNA damage;
Acrylamide;
Testis;
Single cell gel electrophoresis
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know DNA damage effect in testicular cells in mice induced by acrylamide through three exposure routes,intraperitoneal injection,oral exposure and skin exposure.Methods Twenty-four healthy male KM mice were randomly divided into 4 groups,including the blank control group,intraperitoneal injection group,oral exposure group and skin exposure group,6 in each.The exposure dose was 25 mg(/kg?d),for 5 consecutive days,once a day.On the sixth day after the first exposure,the changes of body weight and testis coefficient were observed.The tail length,tail DNA%,tail moment,olive tail moment of testicular cells were detected by single cell gel electrophoresis.Results The body weights of intraperitoneal injection group and oral exposure group decreased significantly (P
