GAS CHROMATOGRAPHIC DETERMINATION OF CHOLESTEROL CONTENT IN EGG YOLK
- VernacularTitle:气相色谱法对蛋黄中胆固醇含量的测定
- Author:
Feng DENG
;
Yingtian ZHOU
- Publication Type:Journal Article
- Keywords:
egg yolk gas liquid chromatography
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A gas chromatographic method was established for determination of cholesterol. The egg yolk was directly saponified with 1ml 40% potassium hydroxide and 20ml ethylene glycol. The unsaponifiable substances were extracted by diethyl ether and then dried with anhydrous sodium sulfate. The extract (2?l) was injected into the gas chromatograph. The average percentage of recovery was 100.0?2.6%. The precision of the method was good, the coefficient of variation was 1.9%. The mean cholesterol content of egg yolk determined by this method was 1247.1?80.1 mg/100g and agreed with the value reported in literature.
