Effects of 17?-estradiol on MCF-7 Cell Proliferation and Regulation of Estrogen Receptor-related Receptor (ERR?)
- VernacularTitle:17?-雌二醇对乳腺癌细胞的增殖作用及其对雌激素受体相关受体的调控作用
- Author:
Yinghua LIU
;
Ning HE
;
Shuqing JIANG
- Publication Type:Journal Article
- Keywords:
Estrogen;
Estrogen receptor-related receptor alpha;
Breast cancer;
Proliferation effect
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the effects of 17?-estradiol on MCF-7 cell proliferation and the regulation of estrogen receptor-related recepto(rERR?).Methods MCF-7 cells were treated with 17?-E2 at the doses of 10-12,10-11,10-10,10-9,10-8,10-7 and 10-6 mol/L for 24 h.Dimethyl sulfoxide were used as the solvent control.MTT method was used to determine inhibitory rate of MCF-7 proliferation,and the cells were treated with 17?-E2 at the doses of 10-10,10-9,10-8 and 10-7 mol/L for 24 h.RT-PCR method was used to detect the expression level of ERR?.Results Cell proliferation were promoted by exposure to 17?-E2 at the doses of 10-12,10-11,10-10,10-9,10-8,10-7 and 10-6 mol/L,the proliferation rate were 112.09%,122.09%,123.42%,120.46%,124.60% and 109.23% respectively,however,cell growth was inhibited at 10-6 mol/L of 17?-E2.The expression of ERR? in MCF-7 cells was upregulated at the doses of 10-10,10-9,10-8 and 10-7 mol/L when cells were treated with 17?-E2 for 24 h.Conclusion In certain dosage range,cell proliferation and expression of ERR? can be promoted by 17?-E2,which suggests that ERR? should play an important role in the process of MCF-7 cells growth.
