Inhibitory Effects of 5-aza-2'-deoxycytidine and Histone Deacetylase Inhibitors on RD-ES Cells in Culture
- VernacularTitle:5-杂氮-2’脱氧胞啶联合组蛋白乙酰基转移酶抑制剂对体外RD-ES细胞的抑制作用
- Author:
Zhe LIU
;
Feng ZHANG
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
5-aza-2'deoxycytidine;
Histone deacetylase;
RD-ES cell
- From:
Journal of Environment and Health
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know the inhibitory effects of 5aza-CdR in combination with HDAC inhibitors on RD-ES cells in culture.Methods This study was to investigate that the HDAC inhibitors (MS-275,Trichostatin-A and depsipeptide)and 5azaCdR inhibition effect on RD-ES cells by MTT.High sensitivity DNA kit was used to detect DNA inhibition rate.RT-PCR method was used to detect the expression of ECAD and TSLC1 for 5aza-CdR joint Depsi on RD-ES cells.Results IC50 of 5aza-CdR for RD-ES cell was 40 ng/ml,for 48 h 50 % DNA inhibition rate,the concentration was approximately 50 ng/ml.IC50 of HDAC inhibitors (MS-275,trichostatin-A,Depsi) for RD-ES cell was 400 ng/ml,2.5 ng/ml and 0.6 ng/ml respectively.The inhibition rate of 5aza-CdR jointed HDAC inhibitors was significantly higher compared with the single used groups (P
