Effects of cobalt and chromium ions on osteoblasts:cytotoxicity and the releasion of RANKL and OPG
- VernacularTitle:钴铬离子对成骨细胞细胞毒性及对其分泌骨保护素及其配体的影响
- Author:
Min DAI
;
Xiaojun YUAN
;
Xigao CHEN
- Publication Type:Journal Article
- Keywords:
cobalt ion;
chromium ion;
osteoblast;
OPG;
RANKL
- From:
Orthopedic Journal of China
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the cytotoxicity of cobalt(Co2+) and chromium(Cr3+)ions on mice osteoblast(MC3T3E1)and the effects on the secretion of RANKL/OPG from osteoblast because of the stimulation of Co2+ and Cr3+ ions. [Methods]The osteoblast in vitri was cultured.The cell viability was assured by MTT test.ELISA method was applied to detect RANKL(receptor activator of nuclear factor-kB ligand),OPG(osteoprotegerin) content in serum supernatant.[Results]Compared to the control,MTT test demonstrated that Co2+ and Cr3+ ions can obviously decrease the cell viability of osteoblast.When osteoblast were exposed to Co2+ and Cr3+ ions for 24 and 48 hours,the releasion of OPG increased by 32.1% and 17.8% as compared with the control(P
