Protective Effect of Aminoguanidine on Pancreatic Islet in Rats
- VernacularTitle:氨基胍对大鼠胰岛保护作用的实验研究
- Author:
Baifeng LI
;
Yongfeng LIU
;
Ying CHENG
;
Jialin ZHANG
- Publication Type:Journal Article
- Keywords:
Cell culture;
Islet transplantation;
Inducible nitric oxide synthase;
Aminoguanidine;
Cytokine
- From:
Chinese Journal of Bases and Clinics in General Surgery
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of inducible nitric oxide synthase(iNOS) inhibitor aminoguanidine on pancreas islets cultured with cytokines TNF-? and IL-1? in rats.Methods Islets isolated from Wistar rats were purified and cultured.According to whether cytokines TNF-?,IL-1? and aminoguanidine were added into the medium respectively or not,islets were divided into 4 groups: cultured with islet only was taken as blank control group,cultured with TNF-?+IL-1? as cytokine group,cultured with aminoguanidine as aminoguanidine group,and cultured with TNF-?+IL-1? and aminoguanidine as aminoguanidine+cytokine group.NO level in culture medium and iNOS activity in islets tissue(Test Kit),apoptosis(TUNEL method) and viability of islets cell(acridine orange/ethidium bromide stain),and the function of islets(insulin release test) were measured.Results Compared with blank control group,the activity of iNOS in islet tissue and level of NO in culture medium increased,and the mass mortality and apoptosis appeared in islet cells,while insulin secretion decreased in cytokine group(P
