The influence of swallowing autoantigen on differentiation,development and function of the bone marrow-derived DC in vitro
- VernacularTitle:吞噬自身抗原对骨髓来源树突状细胞体外分化发育及功能的影响
- Author:
Jun XIE
;
Youkun LIN
;
Hong LUO
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
Lymphocyte;
Autoantigen;
Swallow;
Immune tolerance;
Humoral immune
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes in differentiation,development and function of DCs after mouse bone marrow-derived dendritic cells devouring autoantigen in vitro,to study its impact on the immune status.Methods:Mouse bone marrow cells were cultured in GM-CSF+IL-4 to generate DCs,and the purity of DCs was detected by fluorescence microscope and flow cytometry.It was identified whether DCs could swallow autoantigen by immunohistochemistry and transmission electron microscope;and the changes in CD86,MHCⅡ on DCs after swallowing autoantigen were observed by flow cytometry.The changes in proliferation after the mouse spleen lymphocytes were cultured with the same genetic background of DCs were measured by MTT.The amounts of IL-4,IFN-? secreted by the mouse spleen lymphocytes after stimulation with DCs were measured by ELISA.Results:The purity of DCs was 90.6%.DCs could swallow autoantigen,which induced differentiation dysplasia of the DCs in vitro.Swallowing autoantigen had negative effect on the maturation as well as the potentiation to stimulate proliferation of the mouse spleen lymphocytes with the same genetic background.Secretion of IFN-? by stimulated lymphocytes decreased,but secretion of IL-4 promoted.Conclusion:The DCs that swallow autoantigen could inhibit imDCs maturation,but promote the immune tolerance and humoral immunity.
