Suppressive effects of hydroxytyrosol on oxidative stress of THP-1 cells stimulated by LPS
- VernacularTitle:羟基酪醇对LPS刺激的THP-1细胞氧化应激的抑制作用
- Author:
Xiaomei ZHANG
;
Lin LIU
;
Laifu ZHONG
- Publication Type:Journal Article
- Keywords:
Hydroxytyrosol;
THP-1 cells;
Oxidative stress;
NF-?B
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether hydroxytyrosol (HT) may ameliorate oxidative stress and nuclear factor kappa B (NF-?B) activation in the lipopolysaccharide (LPS)-stimulated THP-1 cell line.Methods:The intracellular formation of reactive oxygen species (ROS) was detected by 2,7-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe.Intracellular glutathione (GSH) level was estimated by fluorometric methods;Nitric oxide (NO) production was measured as nitrite (a stable metabolite of NO) concentrations.Western blot analysis was used to detect the expressin level of NF-?B.Results:The results showed that treatment of THP-1 cells with HT significantly reduced LPS-stimulated NO production and ROS formation in a concentration-dependent manner.HT at 50 and 100 ?mol/L concentrations increased the GSH level.The specific DNA-binding activities of NF-?B in nuclear extracts from 50 and 100 ?mol/L HT treatments were significantly suppressed.The antioxidant N-acetylcysteine (NAC) also showed the same effects as HT on LPS-induced ROS and NO production,change of GSH level,and NF-?B activation.Conclusion:These findings suggest that HT has antioxidant activity by suppressing intracellular oxidative stress and NF-?B activation in THP-1 cells.
