Ultrasound and microbubbles mediated shRNA transfection silences survivin gene expression
- VernacularTitle:超声及超声微泡联合shRNA质粒对survivin基因的沉默效应
- Author:
Zhiyi CHEN
;
Kun LIANG
;
Jianhua LIU
- Publication Type:Journal Article
- Keywords:
Ultrasound;
Microbubble;
Gene;
urvivin;
RNA interference;
Nonvial vector.
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of the association of ultrasound and microbubble with short hairpin RNA (shRNA) on the expression of survivin mRNA and protein in cervical cancer (HeLa) cell lines.Methods:Three survivin-shRNA expression vectors (pSIREN/S1/S2/S3) were constructed and introduced into HeLa cells.A corresponding negative control vector was constructed (pSIREN/con).The shRNA vectors were added to Hela cells transfected by LipofectamineTM (P+L) or followed by ultrasound exposure with SonoVue microbubble (US+SO).Blank control group,pSIREN/S1 plasmid only,ultrasound exposure only,pSIREN/S1 and ultrasound exposure were used as controls,respectively.The expressions of survivin mRNA and protein were detected by RT-PCR and western blot analysis,respectively.Results:Three recombinant plasmids were successfully constructed.The plasmids were showed to inhibit the expression of survivin gene mRNA and protein efficiently and specifically except for the pSIREN/con,while pSIREN/S3 was significantly lower than other groups (P
