Determination of Environmental Estrogens in Mutton by Gas Chromatography-Mass Spectrometry
- VernacularTitle:羊肉中环境雌激素的气相色谱-质谱测定法
- Author:
Shuhui WANG
;
Hong ZHANG
;
Lixue YANG
- Publication Type:Journal Article
- Keywords:
Gas ghromatography-mass spectrometry;
Environmental estrogens;
Mutton
- From:
Journal of Environment and Health
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a gas chromatography-mass spectrometric method(GC/MS) for simultaneous determination of environmental estrogens including 4-nonylphenol,bishphenol A,diethylstilbestrol and 17?-ethinylestradiol and endogenic estrogens including 17?-estradiol,17?-estradiol,estriol and estrone in mutton. Methods Mutton samples were extracted using a mixture of acetonitrile-ethyl acetate(3∶2,V/V) and cleaned up by florisil cartridge. Derived with pentafluoropropionic anhydride(PFPA) ,the derivatives were determined by GC-MS. Results The linear range of determination was 0.2-5.0 ?g/kg. The intraday relative standard deviations of the peak area were 1.25%—5.85% and the detection limits ranged from 0.024 to 1.40 ?g/kg,and the rates of recovery ranged from 71.5%—111.0% except for 17?-ethinylestradiol( 55.4%) . Conclusion This method is simple,rapid,accurate and applicable to the determination of trace environmental estrogens in mutton
