Determination of Allicin in Ultrafine Garlic Powder by Capillary Gas Chromatograph
- VernacularTitle:超微大蒜粉中大蒜素含量的毛细管气相色谱测定法
- Author:
Yongjun LI
;
Aiying SONG
;
Xiaomin QI
- Publication Type:Journal Article
- Keywords:
Chromatography,gas;
Allicin;
Ultrafine garlic powder
- From:
Journal of Environment and Health
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of allicin(diallyl trisulfide) in the ultrafine garlic powder by gas chromatography(GC) . Methods Allicin injection was extracted by supersonic wave with a mixed solvent hexane-anhydrous ethanol(1:4) in GC by capillary column and detected by hydrogen flame ionization detector(FID) ,using nitrogen as the carrier gas. Results The peak areas were proportional to the allicin concentrations and the standard curve showed good linearity,y=190 282.5x-11 734.5(r=0.999 3) within the range of 0.30～3.00 mg/ml,the average recovery was 84.6%-98.6 % with RSD of 1.85%～3.27%. Conclusion The method is simple,accurate with good repeatability,and it is applicable to the determination of allicin in the ultrafine garlic powder.
