Protective effects of ligustrazine on glutamate-induced neurotoxicity in cultured hippocampal neurons
- VernacularTitle:川芎嗪对谷氨酸损伤海马神经元的保护作用
- Author:
Zhijun BAO
;
Chunlin XIA
- Publication Type:Journal Article
- Keywords:
ligustrazine;
glutamate;
hippocampal neuron
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of ligustrazine on glutamate-induced injury in cultured hippocampal neurons. Methods Primarily cultured hippocampal neurons from fetal rats were incubated with ligustrazine (1 mmol/L) for 12 hours,then glutamate (1 mmol/L) was added for 20 minutes to induce injury. Cell viability was detected by MTT assay,and the change in LDH activity was determined by biochemical method. Finally,the expression of AchE in the cultured neurons was assayed by immuocytochemistry. Results Ligustrazine improved the survival rate of hippocampal neurons injured by glutamate,inhibited the release of LDH from the kytoplasm injured by glutamate. The expression of AchE in the injured neurons was promoted by pretreatment of ligustrazine. Conclusion Ligustrazine can significantly exert protective effects on the hippocampal neuron injury induced by glutamate.
