Effects of rAAV2/1-Acrp30 on NF-?B and adhesion molecule in GK rats with atherosclerosis
- VernacularTitle:rAAV2/1-Acrp30对动脉粥样硬化的GK大鼠NF-?B及黏附分子的影响
- Author:
Huiju ZHONG
;
Ying ZHANG
;
Qiangxiang LI
;
Guo LI
- Publication Type:Journal Article
- Keywords:
adiponectin;
diabetic macroangiopathy;
nuclear factor-?B (NF-?B);
soluble intercellular adhesion molecule (sICAM-1);
soluble vascular cell adhesion molecule (sVCAM-1)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of rAAV2/1-Acrp30 on sICAM-1 and sVCAM-1 level as well as NF-?B expression in GK rats with diabetic arteriosclerosis so as to explore the effect of adiponectin on diabetic macroangiopathy. Methods A total number of 30 atherosclerotic GK rat models were randomly divided into three groups:① Model group one (M1):hind limb intramuscular injection of normal saline; ② Model group two (M2):hind limb intramuscular injection of vacuity virus rAAV2/1; ③ Treatment group (T):hind limb intramuscular injection of rAAV2/1-Acrp30 at a dose of 1?1012mg/L. After 8 weeks' treatment,the rats were killed,and serum sVCAM-1 and sICAM-1 level as well as aortic NF-?Bp65mRNA expression were measured in each group. Aortic NF-?Bp65mRNA expression was measured by RT-PCR.Results Compared with those in control model groups (M1 and M2),sVCAM-1 and sICAM-1 levels were decreased significantly in the treatment (T) group (P
