CULTIVATION OF EPIDERMAL CELLS USING COLLAGEN FILM AND GRAFLING: A PRELIMINARY REPORT
- VernacularTitle:应用胶原膜作底物培养人表皮细胞和移植的初步报告
- Author:
Wei WANG
- Publication Type:Journal Article
- Keywords:
epidermal cell culture;
collagen film;
F46 membrane;
cultured epidermal cell sheet graf;
burn
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The isolation and purification of collagen from pig dermis is described,The collagen was spread on a F46 membrane. Human epidermal cells were cultured on the collagen film, and the epidermal cell sheets cultured for 5-13d were successfully autografted on excised or granulation wounds in 5 cases. Biopsy from cultured epidermal grafted area showed epidermization. These results suggest that collagen can promote the growth and attachment of epidermal cells.
