Effects of sodium nitroprusside (SNP) on TLR4 in U937 induced by LPS
- VernacularTitle:硝普钠对内毒素诱导的U937细胞TLR4表达的影响
- Author:
Xueling WU
;
Yunfeng ZHAO
;
Xingsheng WANG
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
Nitric oxide donor;
LPS;
TLR4;
TNF-?
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of nitric oxide donor,sodium nitroprusside(SNP)on the expression and production of TLR4 in U937 induced by LPS.Methods:U937 was induced to maturation by PMA and then stimulated by LPS.Then the cells were treated with SNP and divided into four groups:control group without stimulation of LPS,LPS group(10 ng/ml LPS+100 ng/ml rhLBP),low dose SNP group and high dose SNP group(50,500 ?mol/L SNP respectly).The mRNA and protein of TLR4 was determined by RT-PCR and Western blot.Results:The mRNA of TLR4 in SNP groups was lower than those in LPS group(0.308?0.050 and 0.138?0.0044 vs 0.342?0.098,P0.05).Conclusion:SNP might have a potential protective role in LPS induced inflammation such as sepsis and acute lung injury through inhibiting the mRNA and protein expression of TLR4.
