Effects of T-2 Toxin on Testosterone Biosynthesis in Primary Cultured Leydig Cells of Mice
- VernacularTitle:T-2毒素对原代培养的小鼠睾丸间质细胞睾酮合成的影响
- Author:
Jianying YANG
;
Yongfa ZHANG
- Publication Type:Journal Article
- Keywords:
T-2 toxin;
Mice;
Leydig cells;
Testosterone
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of T-2 toxin on testosterone biosynthesis in primary cultured Leydig cell derived from the mouse testis.Methods Leydig cells isolated from Kunming male mice were adjusted to 5?105/ml and the purity was identified by the modified 3?-hydroxysteroid dehydrogenase(HSD) staining method.Blank control group(treated with 0 ng/ml hCG and 0 mol/L T-2 toxin),inductive control group(treated with 10 ng/ml hCG and 0 mol/L T-2 toxin),low dose T-2 toxin exposure group(treated with 10 ng/ml hCG and 10-9 mol/L T-2 toxin),middle dose T-2 toxin exposure group(treated with 10 ng/ml hCG and 10-8 mol/L T-2 toxin) and high dose T-2 toxin exposure group(treated with 10 ng/ml hCG and 10-7 mol/L T-2 toxin) were designed,respectively.The testosterone level was measured after 24 h of incubation.Results After 24 hours culture in liquid medium contained serum,the fresh isolated Leydig cells grew well and the purity exceeded 90%.Through 10 ng/ml hCG induce,the testosterone level of Legdig cells increased significantly and the difference compared to blank control was of statistical sense(P
