Rapid Determination of Five Sweeteners and Two Preservatives in Milk Beverage by HPLC
- VernacularTitle:乳饮料中5种甜味剂和2种防腐剂的高效液相色谱快速测定法
- Author:
Lin LU
;
Yanhong GAO
;
Xiumin XU
- Publication Type:Journal Article
- Keywords:
Chromatography,liquid;
Sweetener;
Preservative
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid test method for the determination of five sweeteners and two preservatives in the milk beverage by HPLC.Methods Trough a set of pretreatment such as dilution,protein precipitation,high-speed centrifugation,the samples were tested.With NUCLEODUR C18 RP-column separation,phosphate buffer(pH=5.0) and acetonitrile were used as the mobile phase,gradient elution analysis was conducted,the detective wavelength was 205nm.Results The separation of one sample was achieved within 15 min,five sweeteners and two preservatives could be well separated,the linear range was 5.00-100.0 mg/L,r≥0.9993,RSD was 1.5%-4.2%(n=6),the average recovery rate was 85.2%-108%.Conclusion This method is simple and quick with better reproducibility and selectivity,it is an effective way to determine sweeteners and preservatives in milk beverage.