Toll-like receptor expression pattern in bovine monocytes-derived macrophages and the influence by Lipopolysaccharid stimulation
- VernacularTitle:脂多糖刺激对牛单核细胞由来的巨噬细胞Toll样受体表达的影响
- Author:
Yijie GUO
;
Sachi TANAKA
;
Guoqi ZHAO
- Publication Type:Journal Article
- Keywords:
Monocytes-derive macrophages;
Bovine;
TLR;
Innate immunity
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine Toll-like receptor expression pattern in monocytes-derived macrophages by Lipopolysaccharid(LPS) stimulation.Methods:Jugular venous blood was collected from three Japanese calves,and then the peripheral blood mononuclear cells(PBMC),were isolated,and cultured for 7 days to collect monocytes-derived macrophages in Repcell and the cells were stimulated with LPS for 24 h to analyze the mRNA expression pattern of TLR in monocytes-derived macrophages.Results:The mRNA expressions of IL-6,TNF-? and IL-1? were significantly increased after LPS stimulation(P
